參數(shù)資料
型號(hào)： S7802
廠商： Electronic Theatre Controls, Inc.
英文描述： CpG WIZ Prader-Willi/Angelman Amplification Kit
中文描述： 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒
文件頁(yè)數(shù)： 25/32頁(yè)
文件大小： 386K
代理商： S7802
21
Recommendations: see Sec. VI.
Appendix,
Laboratory Setup and Precautions.
a. Use fresh aliquots of every PCR component (i.e. dNTPs, buffer, etc.)
b. Use separate sets of pipettors for pre- vs. post-amplification liquid
dispensing.
c. Devote a work area to pre- and post-amplification procedures.
d. Always use aerosol-resistant pipette tips.
e. Always use a clean labcoat and gloves.
W primer set produces an amplification product in some or
all experimental samples, in addition to an amplification
product from the U or M primer set.
Potential Problem: Chemical modification of the experimental DNA
sample(s) is incomplete.
Recommendation:
This will not jeopardize the validity of the assay as long as a product is also
produced using the U or M primer set. The PCR product produced with the W
primer set is always smaller than that produced with either U or M.
The only lanes containing a PCR product are from those
DNA samples (experimental and control) amplified with
the W primer set.
Potential Problem: Chemical modification of the experimental DNA
samples did not work.
Recommendation:
If using the CpGenome DNA Modification Kit (S7820), check the
troubleshooting section of the manual.
CpG WIZ Prader-Willi/Angelman (S7806) Amplification Kit
There is no visual evidence of products in any lane.
Potential Problem: PCR amplification is not initiated.
Recommendations:
a. Confirm that all PCR components were added to the reaction tube.
b. Confirm that the time and temperature settings on the thermocycler match
those described in this manual.
相關(guān)PDF資料
PDF描述
S7804 CpG WIZ Prader-Willi/Angelman Amplification Kit
S7B-EH BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA
S8040N SCRs (1 A to 70 A)
S8040N SCRs
S8040R SCRs
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