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參數(shù)資料
| 型號： | S7802 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進Prader-Willi/Angelman擴增試劑盒 |
| 文件頁數(shù)： | 24/32頁 |
| 文件大?。?/td> | 386K |
| 代理商： | S7802 |
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20
V.
T
ROUBLESHOOTING
CpG WIZ p16 (S7800), p15 (S7802) and E-cadherin (S7804)
Amplification Kits
There is no visual evidence of products in any lane.
Potential Problem: PCR amplification is not initiated.
Recommendations:
a. Confirm that all PCR components were added to the reaction tube.
b. Confirm that the time and temperature settings on the thermocycler match
those described in this manual.
c. If performing "hot start" PCR using a "hot start" enzyme, verify the initial
denaturation/activation time of 12 minutes at 95°C.
d. For all other "hot start" methods, confirm the proper use of the reagents.
e. Confirm that the PCR polymerase is still active.
f.
Confirm that no additional Mg
2+
was added to the PCR reaction mix.
g. The optimal annealing temperature is 60°C. If items #a-f have not remedied
the problem, re-optimize annealing conditions to suit your amplification
instrument.
No amplification product is generated in the experimental
samples using U, M and W primer sets, but products of the
correct size are observed with the control samples.
Potential Problem #1: Experimental DNA samples were degraded
prior to chemical modification.
Recommendation:
Purify the genomic DNA again and repeat the chemical modification.
Potential Problem #2: Chemically modified experimental DNA
samples were stored for more than two months prior to PCR.
Recommendation:
Repeat the chemical modification on new genomic DNA samples.
U or M primer sets are producing bands in all samples,
including the "no DNA" controls.
Potential Problem: PCR reagents are contaminated with
amplification products.
相關PDF資料 |
PDF描述 |
|---|---|
| S7804 | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
相關代理商/技術參數(shù) |
參數(shù)描述 |
|---|---|
| S7804 | 制造商:未知廠家 制造商全稱:未知廠家 功能描述:CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
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