參數(shù)資料
型號(hào)： S7802
廠商： Electronic Theatre Controls, Inc.
英文描述： CpG WIZ Prader-Willi/Angelman Amplification Kit
中文描述： 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒
文件頁(yè)數(shù)： 20/32頁(yè)
文件大?。?/td> 386K
代理商： S7802
16
The amount of reagents required in each reaction is:
10X Universal PCR buffer
2.5 mM dNTP Mix**
P or M primers (white, red)
"Hot start" enzyme (5 U/μL)
dH
2
O
Template DNA (50 ng/μL)
2.5 μL
2.0 μL
2.0 μL
0.1 μL (0.5 U)
16.4 μL
23.0 μL
2.0 μL
TOTAL VOLUME
25.0 μL
** Upon first use, make aliquots of 2.5 mM dNTPs, which should be freeze-
thawed no more than 5 times.
c. Aliquot 23 μL of each "master mix" (white and red) into corresponding PCR
tubes.
d. Add:
2 μL of water to the no DNA control tube.
2 μL of modified sample DNA to each of the sample tubes.
2 μL of modified normal DNA control to each control tube.
e. Place tubes in the thermocycler block, and perform PCR under the following
conditions:
Denature:
for "hot start" enzyme check manufacturer's specifications
Then, perform 35 cycles of the following conditions:
denature
anneal
extend
95°C / 30 seconds
62°C / 30 seconds
72°C / 30 seconds
Final Extension:
72°C /10 minutes
f. Remove the tubes from the thermocycler block. From this point on, it is
important to designate separate pipettors and work areas for amplified vs.
unamplified samples. This prevents carry-over contamination of future DNA
samples with the amplified product.
相關(guān)PDF資料
PDF描述
S7804 CpG WIZ Prader-Willi/Angelman Amplification Kit
S7B-EH BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA
S8040N SCRs (1 A to 70 A)
S8040N SCRs
S8040R SCRs
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