- 您現(xiàn)在的位置：買賣IC網(wǎng) > PDF目錄374671 > S7802 (Electronic Theatre Controls, Inc.) CpG WIZ Prader-Willi/Angelman Amplification Kit PDF資料下載
參數(shù)資料
| 型號： | S7802 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒 |
| 文件頁數(shù)： | 19/32頁 |
| 文件大小： | 386K |
| 代理商： | S7802 |
第1頁第2頁第3頁第4頁第5頁第6頁第7頁第8頁第9頁第10頁第11頁第12頁第13頁第14頁第15頁第16頁第17頁第18頁當(dāng)前第19頁第20頁第21頁第22頁第23頁第24頁第25頁第26頁第27頁第28頁第29頁第30頁第31頁第32頁
15
Lanes 7-8
Experimental sample 4 with P and M primers
Lanes 9-10
Experimental sample 5 with P and M primers
Lane 11
Chemically modified normal control DNA with P primers
Lane 12
Chemically modified normal control DNA with M primers
Lanes 13-14
No DNA Control with P and M primers
Amplification Protocol
To prevent PCR contamination, read Sec. VI.
Appendix,
Laboratory Setup and
Precautions
before beginning.
STEP 1.
Modification
Prior to performing PCR with the primer sets in the CpG WIZ Prader-
Willi/Angelman Amplification Kit, the DNA samples must undergo bisulfite
modification. CpGenome DNA Modification Kit (S7820), contains the
reagents necessary to perform the modification.
STEP 2. Amplification
a. Determine the number of assays to be run in the experiment: run two
amplification reactions for each experimental DNA sample plus four control
reactions per each set of methylation assays (see Sec. III.
Protocols,
Experimental Design
).
b. Prepare two (2) "master mixes" which correspond to the 2 possible primer
sets P and M (primer cap colors-white and red) by mixing all the reagents
outlined below
except
for the template DNA.
To analyze five experimental samples with appropriate controls, use the
following amount of "master mixes" of each color: 7 tubes X 23.0 μL = 161 μL,
plus 10% of that volume to adjust for pipetting error. Multiply the volume of
each reagent listed below by 7 and add 10% for each "master mix" (white and
red). Thaw all reagents and store on ice while creating the "master mixes".
相關(guān)PDF資料 |
PDF描述 |
|---|---|
| S7804 | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
參數(shù)描述 |
|---|---|
| S7804 | 制造商:未知廠家 制造商全稱:未知廠家 功能描述:CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
發(fā)布緊急采購，3分鐘左右您將得到回復(fù)。