參數(shù)資料
型號(hào)： S7802
廠商： Electronic Theatre Controls, Inc.
英文描述： CpG WIZ Prader-Willi/Angelman Amplification Kit
中文描述： 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒
文件頁(yè)數(shù)： 15/32頁(yè)
文件大?。?/td> 386K
代理商： S7802
11
To analyze five experimental samples with appropriate controls, use the
following amount of "master mixes" of each color: 7 tubes X 23.0 μL = 161 μL,
plus 10% of that volume to adjust for pipetting error. Multiply the volume of
each reagent listed below by 7 and add 10% for each "master mix" (white, red
and green). Thaw all reagents and store on ice while creating the "master
mixes". The amount of reagents required in each reaction is:
10X Universal PCR buffer
2.5 mM dNTP Mix**
U, M or W primers (white, red and green)
TaKaRa
Taq
or "hot start" enzyme (5 U/μL)
dH
2
O
2.5 μL
2.5 μL
1.0 μL
0.2 μL (1 Unit)
16.8 μL
23.0 μL
Template DNA (50 ng/μL)
TOTAL VOLUME
2.0 μL
25.0 μL
** Upon first use, make aliquots of 2.5 mM dNTPs, which should be freeze-
thawed no more than 5 times.
c. Aliquot 23 μL of each "master mix" (white, red and green) into
corresponding PCR tubes.
d. Add:
2 μL of water to the no DNA control tube.
2 μL of modified sample DNA to each of the sample tubes.
2 μL of corresponding DNA controls (modified U and M, unmodified W) to
each control tube.
e. Place tubes in the thermocycler block, and perform PCR under the following
conditions:
Denature:
for Taq Polymerase
for "hot start" enzyme check manufacturer's specifications
95°C / 5 minutes
Then, perform 35 cycles of the following conditions:
denature
anneal
extend
95°C / 45 seconds
60°C / 45 seconds
72°C / 60 seconds
相關(guān)PDF資料
PDF描述
S7804 CpG WIZ Prader-Willi/Angelman Amplification Kit
S7B-EH BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA
S8040N SCRs (1 A to 70 A)
S8040N SCRs
S8040R SCRs
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參數(shù)描述
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