Polysciences, Inc.
Active: Jan/2007
Data Sheet #741
1
Page 4 of 6
TECHNICAL DATA SHEET 741
Should any of our materials fail to perform to our specifications, we will be pleased to provide replacements or return the purchase price. We solicit your inquiries concerning all needs for life sciences work. The information
given in this bulletin is to the best of our knowledge accurate, but no warranty is expressed or implied. It is the user’s responsibility to determine the suitability for their own use of the products described herein, and since
conditions of use are beyond our control, we disclaim all liability with respect to the use of any material supplied by us. Nothing contained herein shall be construed as a recommendation to use any product or to practice
any process in violation of any law or any government regulation.
Microring
AC is a filter paper ring used for the identification of Gram-positive anaerobic cocci and some microaerophilic cocci. It has 3
individual printed tips containing various antimicrobial reagents. The resistance or sensitivity patterns can be used to help identify
anaerobic cocci. In particular, P. anaerobius can be readily picked out by it’s sensitivity to SPS and P. magnus can be distinguished
from P. micros by its resistance to Novobiocin.
Microring
AC
Cat. #24654
(50 rings/pkg)
Procedure
1. Spread sample of test organism onto a plate containing anaerobic agar.
2. Using sterile forceps, carefully place Microring
AC in the center of the plate.
Press each tip gently to ensure contact with surface.
3. Incubate overnight at 37° C under anaerobic conditions.
4. Following incubation, growth patterns around each tip are read
and used to identify the organism.
Code
Color
Antibiotic
Amount
MZ
SPS
White
Pink
Metronidazole
Sodium Polyanethol
Sulphonate
Novobiocin
5ug
1mg
NO
Blue
5ug
Species
Metronidazole
Novobiocin
SPS
Indole
Fatty Acids from PYG broth*
Peptococcus niger
Peptostreptococcus asaccharolyticus
Peptostreptococcus indolicus
Peptostreptococcus magnus
Anaerococcus prevotil
Peptostreptococcus anaerobius
Peptostreptococcus micros
Peptostreptococcus productus
Peptostreptococcus tetradius
Atopbium parvulum
Staphylococcus saccharolyticus
S
S
S
S
S
S
S
S
S
S
R
R
R
R
R
S
S
S
R
R
R
R
R
S
R
R
R
R
R
-
+
+
-
-
-
-
-
-
-
-
B,C,iv,a
B,(A,p,l)
B,(A,p,l)
A
B,A,(L),p
IC,A,(iv,ib,b)
A(s)
A,l,s
L,B(A,p)
L,a
F/A
S
R
R/S
Other microaerophilic streptococci
Streptococcus intermedius
Gemella morbillorum
Streptococcus constellatus
R
R
R
S
S
S
R
R
R
-
-
-
L,a
L,a
L,a
Microring
AC Identification
S = Suseptibility, R = Resistant, R/S = aerotolerant strains resistant
(a) Occasional strains may give a zone size >12mm, but these organisms are easily distinguishable from
Peptostreptococcus anaerobius
as they are resistant to Novobicin.
(b) Occasional strains may give a small zone to SPS <12mm in diameter.
* Gas-liquid chromotography analysis of fatty acid products in Peptone Yeast extract Glucose (PYG) broth.
Capital letters indicate major metabolic products, small letters indicate minor products and parentheses indicate variable reaction.
Storage & Handling
Store Microring
AN at 2-8°C
Rings maintain a shelf life of 12 months from manufacture date.